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Single-strand specific nuclease enhances accuracy of error-corrected sequencing and improves rare mutation-detection sensitivity.

Archives of toxicology - 1 Jan 2022

Otsubo Yuki, Matsumura Shoji, Ikeda Naohiro, Yamane Masayuki

Abstract excerpt

Error-corrected sequences (ECSs) that utilize double-stranded DNA sequences are useful in detecting mutagen-induced mutations. However, relatively higher frequencies of G:C > T:A (1 × 10-7 bp) and G:C > C:G (2 × 10-7 bp) errors decrease the accuracy of detection of rare G:C mutations (approximately 10-7 bp). Oxidized guanines in single-strand (SS) overhangs generated after shearing could serve as the source of...

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PMID: 34767040
DOI: 10.1007/s00204-021-03185-y
PMCID: PMC8748355

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