Article
Genomic background selection to reduce the mutation load after random mutagenesis.
Scientific reports - 30 Sept 2021
Karunarathna Nirosha L, Patiranage Dilan S R, Harloff Hans-Joachim, Sashidhar Niharika, Jung Christian
Abstract excerpt
Random mutagenesis is a standard procedure to increase allelic variation in a crop species, especially in countries where the use of genetically modified crops is limited due to legal constraints. The chemical mutagen EMS is used in many species to induce random mutations throughout the genome with high mutation density. The major drawback for functional analysis is a high background mutation load in a single...
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