Article
A mutant Brassica napus (canola) population for the identification of new genetic diversity via TILLING and next generation sequencing.
PloS one - 1 Jan 2013
Gilchrist Erin J, Sidebottom Christine H D, Koh Chu Shin, Macinnes Tanya, Sharpe Andrew G, Haughn George W
Abstract excerpt
We have generated a Brassica napus (canola) population of 3,158 EMS-mutagenised lines and used TILLING to demonstrate that the population has a high enough mutation density that it will be useful for identification of mutations in genes of interest in this important crop species. TILLING is a reverse genetics technique that has been successfully used in many plant and animal species. Classical TILLING involves...
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