Article
A mutation screening platform for rapeseed (Brassica napus L.) and the detection of sinapine biosynthesis mutants.
TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik - 1 Mar 2012
Harloff Hans-Joachim, Lemcke Susanne, Mittasch Juliane, Frolov Andrej, Wu Jian Guo, Dreyer Felix, Leckband Gunhild, Jung Christian
Abstract excerpt
We developed two mutant populations of oilseed rape (Brassica napus L.) using EMS (ethylmethanesulfonate) as a mutagen. The populations were derived from the spring type line YN01-429 and the winter type cultivar Express 617 encompassing 5,361 and 3,488 M(2) plants, respectively. A high-throughput screening protocol was established based on a two-dimensional 8× pooling strategy. Genes of the sinapine biosynthesis...
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