Article
Direct access to millions of mutations by whole genome sequencing of an oilseed rape mutant population.
The Plant journal : for cell and molecular biology - 1 Feb 2023
Jhingan Srijan, Kumar Avneesh, Harloff Hans-Joachim, Dreyer Felix, Abbadi Amine, Beckmann Katrin, Obermeier Christian, Jung Christian
Abstract excerpt
Induced mutations are an essential source of genetic variation in plant breeding. Ethyl methanesulfonate (EMS) mutagenesis has been frequently applied, and mutants have been detected by phenotypic or genotypic screening of large populations. In the present study, a rapeseed M2 population was derived from M1 parent cultivar 'Express' treated with EMS. Whole genomes were sequenced from fourfold (4×) pools of...
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