Article
Discovery and engineering of enhanced SUMO protease enzymes.
The Journal of biological chemistry - 24 Aug 2018
Lau Yue-Ting K, Baytshtok Vladimir, Howard Tessa A, Fiala Brooke M, Johnson JayLee M, Carter Lauren P, Baker David, Lima Christopher D, Bahl Christopher D
Abstract excerpt
Small ubiquitin-like modifier (SUMO) is commonly used as a protein fusion domain to facilitate expression and purification of recombinant proteins, and a SUMO-specific protease is then used to remove SUMO from these proteins. Although this protease is highly specific, its limited solubility and stability hamper its utility as an in vitro reagent. Here, we report improved SUMO protease enzymes obtained via two...
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