Article
Rab29 Fast Exchange Mutants: Characterization of a Challenging Rab GTPase.
Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021
Gomez Rachel C, Vides Edmundo G, Pfeffer Suzanne R
Abstract excerpt
Rab29 has been implicated in multiple membrane trafficking processes with no described effectors or regulating proteins. Its fast nucleotide exchange rate and inability to bind GDI in cytosol make it a unique and poorly understood Rab. Because the conventional, "GTP-locked" Rab mutation does not have the desired effect in Rab29, we present here the use of a fluorescence-based assay to characterize novel Rab29...
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