Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Single-Amplicon Multiplex Real-Time Reverse Transcription-PCR with Tiled Probes To Detect SARS-CoV-2 spike Mutations Associated with Variants of Concern.

Journal of clinical microbiology - 18 Nov 2021

Babiker Ahmed, Immergluck Katherine, Stampfer Samuel D, Rao Anuradha, Bassit Leda, Su Max, Nguyen Vi, Stittleburg Victoria, Ingersoll Jessica M, Bradley Heath L, Mavigner Maud, Schoof Nils, Kraft Colleen S, Chahroudi Ann, Schinazi Raymond F, Martin Greg S, Piantadosi Anne, Lam Wilbur A, Waggoner Jesse J

Abstract excerpt

To provide an accessible and inexpensive method to surveil for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutations, we developed a multiplex real-time reverse transcription-PCR (rRT-PCR) assay, the Spike single-nucleotide polymorphism (SNP) assay, to detect specific mutations in the spike receptor binding domain. A single primer pair was designed to amplify a 348-bp region of spike, and probes...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to COVID-19
Share to Mutation

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Real-Time Polymerase Chain Reaction

Share to Reverse Transcription

Share to RNA, Viral

Share to SARS-CoV-2

Share to SARS-CoV-2 and COVID-19 Research

Share to SARS-CoV-2 detection and testing

Source

Open in PubMed Open full text

Identifiers

PMID: 34432488
DOI: 10.1128/JCM.01446-21
PMCID: PMC8601233

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Single-Amplicon Multiplex Real-Time Reverse Transcription-PCR with Tiled Probes To Detect SARS-CoV-2 spike Mutations Associated with Variants of Concern.PMID 34432488Read ↗

Real-time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with Variants of ConcernSemantic proximity Read ↗

Surveillance of SARS-CoV-2 variants of concern by identification of single nucleotide polymorphisms in the spike protein by a multiplex real-time PCR.Semantic proximity Read ↗

Rapid detection of SARS-CoV-2 variants of concern by single nucleotide polymorphism genotyping using TaqMan assays.Semantic proximity Read ↗

Development of an efficient Sanger sequencing-based assay for detecting SARS-CoV-2 spike mutations.Semantic proximity Read ↗

Mutation-Specific SARS-CoV-2 PCR Screen: Rapid and Accurate Detection of Variants of Concern and the Identification of a Newly Emerging Variant with Spike L452R Mutation.Semantic proximity Read ↗

High-Integrity Sequencing of Spike Gene for SARS-CoV-2 Variant Determination.Semantic proximity Read ↗

Real-Time RT-PCR Allelic Discrimination Assay for Detection of N501Y Mutation in the Spike Protein of SARS-CoV-2 Associated with B.1.1.7 Variant of Concern.Semantic proximity Read ↗

Mutation-specific SARS-CoV-2 PCR Screen: Rapid and Accurate Detection of Variants of Concern and the Identification of a Newly Emerging Variant with Spike L452R MutationSemantic proximity Read ↗

Efficient SARS-CoV-2 variant detection and monitoring with Spike Screen next-generation sequencing.Semantic proximity Read ↗

SARS-CoV-2 spike gene Sanger sequencing methodology to identify variants of concern.Semantic proximity Read ↗

Targeted Amplification and Genetic Sequencing of the Severe Acute Respiratory Syndrome Coronavirus 2 Surface GlycoproteinSemantic proximity Read ↗

Multiplex SARS-CoV-2 Genotyping PCR for Population-Level Variant Screening and Epidemiologic SurveillanceSemantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.