Article
Surveillance of SARS-CoV-2 variants of concern by identification of single nucleotide polymorphisms in the spike protein by a multiplex real-time PCR.
Journal of virological methods - 1 Feb 2022
Gomes Laksiri, Jeewandara Chandima, Jayadas Tibutius Pramanayagam, Dissanayake Osanda, Harvie Michael, Guruge Dinuka, Withanage Vigeetha, Mahesh Pasyodun Koralage Buddhika, Rajapakse Wajira, Ramachandran Rajanthi, Dharmarajan Venoden, Pathiraja Indika, Sanjeewani Anusha, Bandara Priyantha, Nanayakkara Geetha, Francis Vaithehi Rajeevan, Kithsiri Ananda, Edirisinghe Dilini Anushika, De Silva Kaushalya, Wijayamuni Ruwan, Ogg Graham S, Waggoner Jesse, Malavige Gathsaurie Neelika
Abstract excerpt
BACKGROUND: Although active surveillance SARS-CoV-2 variants of concern (VOCs) is required for proper outbreak control measures, many lower income countries find it challenging to detect VOCs by carrying genomic sequencing alone, due to limited resources. METHODS: VOCs can also be identified by the unique mutations in the spike protein by real-time PCR that detect these single nucleotide polymorphisms (SNPs). We...
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