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Article

High-Integrity Sequencing of Spike Gene for SARS-CoV-2 Variant Determination.

International journal of molecular sciences - 17 Mar 2022

Liao Yu-Chieh, Chen Feng-Jui, Chuang Min-Chieh, Wu Han-Chieh, Ji Wan-Chen, Yu Guann-Yi, Huang Tsi-Shu

Abstract excerpt

For tiling of the SARS-CoV-2 genome, the ARTIC Network provided a V4 protocol using 99 pairs of primers for amplicon production and is currently the widely used amplicon-based approach. However, this technique has regions of low sequence coverage and is labour-, time-, and cost-intensive. Moreover, it requires 14 pairs of primers in two separate PCRs to obtain spike gene sequences. To overcome these...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 35328676
DOI: 10.3390/ijms23063257
PMCID: PMC8954144

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