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SARS-CoV-2 spike gene Sanger sequencing methodology to identify variants of concern.

BioTechniques - 1 Feb 2023

Alhamlan Fatimah S, Bakheet Dana M, Bohol Marie F, Alsanea Madain S, Alahideb Basma M, Alhadeq Faten M, Alsuwairi Feda A, Al-Abdulkareem Maha A, Asiri Mohamed S, Almaghrabi Reem S, Altamimi Sarah A, Mutabagani Maysoon S, Althawadi Sahar I, Al-Qahtani Ahmed A

Abstract excerpt

The global demand for rapid identification of circulating SARS-CoV-2 variants of concern has led to a shortage of commercial kits. Therefore, this study aimed to develop and validate a rapid, cost-efficient genome sequencing protocol to identify circulating SARS-CoV-2 (variants of concern). Sets of primers flanking the SARS-CoV-2 spike gene were designed, verified and then validated using 282 nasopharyngeal...

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Identifiers

PMID: 36794696
DOI: 10.2144/btn-2021-0114
PMCID: PMC9937032

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