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Rapid detection of SARS-CoV-2 variants of concern by single nucleotide polymorphism genotyping using TaqMan assays.

Diagnostic microbiology and infectious disease - 1 Dec 2022

Velu Priya, Cong Lin, Rand Sophie, Qiu Yuqing, Zhang Zhengmao, Zhang Jianxuan, Guo Jianfen, Ruggiero Phyllis, Sukhu Ashley, Fauntleroy Kathy, Imada Eddie, Zanettini Claudio, Brundage David, Westblade Lars, Marchionni Luigi, Cushing Melissa M, Rennert Hanna

Abstract excerpt

We evaluated the performance of SARS-CoV-2 TaqMan real-time reverse-transcription PCR (RT-qPCR) assays (ThermoFisher) for detecting 2 nonsynonymous spike protein mutations, E484K and N501Y. Assay accuracy was evaluated by whole genome sequencing (WGS). Residual nasopharyngeal SARS-CoV-2 positive samples (N = 510) from a diverse patient population in New York City submitted for routine SARS-CoV-2 testing during...

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Identifiers

PMID: 36122486
DOI: 10.1016/j.diagmicrobio.2022.115789
PMCID: PMC9392658

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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