Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

High allele discrimination in the typing of single nucleotide polymorphisms of miRNA.

Bioorganic & medicinal chemistry - 15 Sept 2021

Takei Fumie, Akiyama Misaki, Dateki Minori

Abstract excerpt

MicroRNAs (miRNAs) belonging to the same family have similar sequences and are difficult to identify. Herein, we report the reverse transcription-hairpin-probe-polymerase chain reaction (RT-Hpro-PCR) technique, which utilises a reverse transcription (RT) primer containing a 5'-end deoxyribonucleic acid (DNA) tag, to detect miRNAs with similar sequences. This strategy follows a two-step RT-PCR method using 6-7-mer...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to MicroRNAs
Share to Polymorphism, Single Nucleotide

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Reverse Transcriptase Polymerase Chain Reaction

Source

Identifiers

PMID: 34419822
DOI: 10.1016/j.bmc.2021.116363

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

High allele discrimination in the typing of single nucleotide polymorphisms of miRNA.PMID 34419822Read ↗

Single-nucleotide polymorphisms among microRNA: big effects on cancer.Semantic proximity Read ↗

A dual discrimination mode for improved specificity towards let-7a detection via a single-base mutated padlock probe-based exponential rolling circle amplification.Semantic proximity Read ↗

[Progress of polymorphism in microRNA and microRNA target sites].Semantic proximity Read ↗

microRNA single polynucleotide polymorphism influences on microRNA biogenesis and mRNA target specificity.Semantic proximity Read ↗

Polymorphisms in microRNA targets: a gold mine for molecular epidemiology.Semantic proximity Read ↗

miRNova: A Next-Generation Platform for Ultra-Precise and Highly Specific MicroRNA Quantification Integrating a Tailored Stem-Loop RT-qPCR and a Robust Analytical FrameworkSemantic proximity Read ↗

PRICE: direct and robust detection of microRNAs at single-nucleotide resolution.Semantic proximity Read ↗

Principles of miRNA-mRNA interactions: beyond sequence complementarity.Semantic proximity Read ↗

dPORE-miRNA: polymorphic regulation of microRNA genes.Semantic proximity Read ↗

Application of the C4'-alkylated deoxyribose primer system (CAPS) in allele-specific real-time PCR for increased selectivity in discrimination of single nucleotide sequence variants.Semantic proximity Read ↗

[Methods for detecting single nucleotide polymorphisms: allele-specific PCR and hybridization with oligonucleotide probe].Semantic proximity Read ↗

Ribonucleotide-Modified Primer Overlapping Blocker Elongation (PROBE) Assay for Highly Specific Mutation Detection.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.