Article

miRNova: A Next-Generation Platform for Ultra-Precise and Highly Specific MicroRNA Quantification Integrating a Tailored Stem-Loop RT-qPCR and a Robust Analytical Framework

2026-04-04

Abstract excerpt

MicroRNAs (miRNAs) are ultra-short RNA molecules characterized by high sequence homology, frequent post-transcriptional modifications, and typically low abundance, particularly in circulating biofluids. These inherent biological features present substantial technical challenges for RT-qPCR– based quantification. Consequently, the development of miRNA RT-qPCR assays has required architectural adaptations at the rev...

Topics

Open a Topic to create a Post that cites this publication.

Identifiers and source

Literature Corpus work: 653b454c-bd67-544c-af85-80493ab6655d
DOI: 10.64898/2026.04.01.715903

Open publication

Related research

Semantic proximity does not establish scientific evidence.

Click a neighbor to travelStep 1 · 12 closest

miRNova: A Next-Generation Platform for Ultra-Precise and Highly Specific MicroRNA Quantification Integrating a Tailored Stem-Loop RT-qPCR and a Robust Analytical FrameworkDOI 10.64898/2026.04.01.715903Read

miRquad: first-in-class dPCR multiplex TaqMan™ Advanced RUO assay for microRNA detection in head and neck cancerSemantic proximity Read

Assessment of salivary microRNA by RT-qPCR: Challenges in data interpretation for clinical diagnosisSemantic proximity Read

A Novel MiRNA Detection System Combining TWJ-SDA, Multistep L-TEAM, and CRISPR-Cas3Semantic proximity Read

Dumbbell-PCR for Discriminative Quantification of a Small RNA Variant.Semantic proximity Read

Development of rapid and simple Xeno Nucleic Acid (XNA) sensor-based microRNA detection method for Parkinson’s disease diagnosticsSemantic proximity Read

An extension to: Systematic assessment of commercially available low-input miRNA library preparation kitsSemantic proximity Read

High allele discrimination in the typing of single nucleotide polymorphisms of miRNA.Semantic proximity Read

Extracellular microRNA 3’ end modification across diverse body fluidsSemantic proximity Read

qPCR Guru, a free browser-based platform, strengthens microRNA analyses using full-curve Cq estimationSemantic proximity Read

Hairpin-Functionalized Gold Nanoparticles as an Adaptable Platform for Detecting MicroRNA SignaturesSemantic proximity Read

A dual discrimination mode for improved specificity towards let-7a detection via a single-base mutated padlock probe-based exponential rolling circle amplification.Semantic proximity Read

TUCL-Seq for miRNA single molecule sequencingSemantic proximity Read