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Article

An in vivo target mutagenesis system for multiple hosts.

Trends in biotechnology - 1 Aug 2025

Yin Dong, Pang Qingxiao, Yuan Yingbo, Su Tianyuan, Liu Mengmeng, Wang Qian, Hou Jin, Qi Qingsheng

Abstract excerpt

In vivo target mutagenesis is a powerful approach to accelerate protein evolution. However, current approaches have been primarily developed in conventional organisms, limiting their capacity to evolve proteins with subtle variations across non-conventional host species. Here, we design an in vivo target mutagenesis system for multiple hosts (ITMU) utilizing the broad host-range plasmid RSF1010 replication...

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PMID: 40345898
DOI: 10.1016/j.tibtech.2025.04.005

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