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Article

Amplicon Sequence Variants Artificially Split Bacterial Genomes into Separate Clusters.

mSphere - 25 Aug 2021

Schloss Patrick D

Abstract excerpt

Amplicon sequencing variants (ASVs) have been proposed as an alternative to operational taxonomic units (OTUs) for analyzing microbial communities. ASVs have grown in popularity, in part because of a desire to reflect a more refined level of taxonomy since they do not cluster sequences based on a distance-based threshold. However, ASVs and the use of overly narrow thresholds to identify OTUs increase the risk of...

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Identifiers

PMID: 34287003
DOI: 10.1128/mSphere.00191-21
PMCID: PMC8386465

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