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Article

Exact sequence variants should replace operational taxonomic units in marker-gene data analysis.

The ISME journal - 1 Dec 2017

Callahan Benjamin J, McMurdie Paul J, Holmes Susan P

Abstract excerpt

Recent advances have made it possible to analyze high-throughput marker-gene sequencing data without resorting to the customary construction of molecular operational taxonomic units (OTUs): clusters of sequencing reads that differ by less than a fixed dissimilarity threshold. New methods control errors sufficiently such that amplicon sequence variants (ASVs) can be resolved exactly, down to the level of...

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PMID: 28731476
DOI: 10.1038/ismej.2017.119
PMCID: PMC5702726

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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