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Article

Intragenomic heterogeneity of 16S rRNA genes causes overestimation of prokaryotic diversity.

Applied and environmental microbiology - 1 Oct 2013

Sun Dong-Lei, Jiang Xuan, Wu Qinglong L, Zhou Ning-Yi

Abstract excerpt

Ever since Carl Woese introduced the use of 16S rRNA genes for determining the phylogenetic relationships of prokaryotes, this method has been regarded as the "gold standard" in both microbial phylogeny and ecology studies. However, intragenomic heterogeneity within 16S rRNA genes has been reported in many investigations and is believed to bias the estimation of prokaryotic diversity. In the current study, 2,013...

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PMID: 23872556
DOI: 10.1128/AEM.01282-13
PMCID: PMC3811346

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