Article
Mastering methodological pitfalls for surviving the metagenomic jungle.
BioEssays : news and reviews in molecular, cellular and developmental biology - 1 Aug 2013
Delmont Tom O, Simonet Pascal, Vogel Timothy M
Abstract excerpt
Metagenomics is a culture- and PCR-independent approach that is now widely exploited for directly studying microbial evolution, microbial ecology, and developing biotechnologies. Observations and discoveries are critically dependent on DNA extraction methods, sequencing technologies, and bioinformatics tools. The potential pitfalls need to be understood and, to some degree, mastered if the resulting data are to...
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