Article
Evaluation of 16S rRNA gene sequencing for species and strain-level microbiome analysis.
Nature communications - 6 Nov 2019
Johnson Jethro S, Spakowicz Daniel J, Hong Bo-Young, Petersen Lauren M, Demkowicz Patrick, Chen Lei, Leopold Shana R, Hanson Blake M, Agresta Hanako O, Gerstein Mark, Sodergren Erica, Weinstock George M
Abstract excerpt
The 16S rRNA gene has been a mainstay of sequence-based bacterial analysis for decades. However, high-throughput sequencing of the full gene has only recently become a realistic prospect. Here, we use in silico and sequence-based experiments to critically re-evaluate the potential of the 16S gene to provide taxonomic resolution at species and strain level. We demonstrate that targeting of 16S variable regions...
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