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High throughput single-cell detection of multiplex CRISPR-edited gene modifications

20 Oct 2020

Abstract excerpt

CRISPR-Cas9 gene editing has transformed our ability to rapidly interrogate the functional impact of somatic mutations in human cancers. Droplet-based technology enables the analysis of Cas9-introduced gene edits in thousands of single cells. Using this technology, we analyze Ba/F3 cells engineered to express single or multiplexed loss-of-function mutations recurrent in chronic lymphocytic leukemia. Our approach...

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PMID: 33081820
DOI: 10.1186/s13059-020-02174-1

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