Article
Novel Escherichia coli active site dnaE alleles with altered base and sugar selectivity.
Molecular microbiology - 1 Sept 2021
Vaisman Alexandra, Łazowski Krystian, Reijns Martin A M, Walsh Erin, McDonald John P, Moreno Kristiniana C, Quiros Dominic R, Schmidt Marlen, Kranz Harald, Yang Wei, Makiela-Dzbenska Karolina, Woodgate Roger
Abstract excerpt
The Escherichia coli dnaE gene encodes the α-catalytic subunit (pol IIIα) of DNA polymerase III, the cell's main replicase. Like all high-fidelity DNA polymerases, pol III possesses stringent base and sugar discrimination. The latter is mediated by a so-called "steric gate" residue in the active site of the polymerase that physically clashes with the 2'-OH of an incoming ribonucleotide. Our structural modeling...
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