Article
Structurally distributed surface sites tune allosteric regulation.
eLife - 16 Jun 2021
McCormick James W, Russo Marielle Ax, Thompson Samuel, Blevins Aubrie, Reynolds Kimberly A
Abstract excerpt
Our ability to rationally optimize allosteric regulation is limited by incomplete knowledge of the mutations that tune allostery. Are these mutations few or abundant, structurally localized or distributed? To examine this, we conducted saturation mutagenesis of a synthetic allosteric switch in which Dihydrofolate reductase (DHFR) is regulated by a blue-light sensitive LOV2 domain. Using a high-throughput assay...
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