Article
Whole-genome synthesis and characterization of viable S13-like bacteriophages.
PloS one - 1 Jan 2012
Liu Yuchen, Han Yonghua, Huang Weiren, Duan Yonggang, Mou Lisha, Jiang Zhimao, Fa Pingping, Xie Jun, Diao Ruiying, Chen Yuanbin, Ye Yiwang, Yang Ruilin, Chen Jing, Sun Xiaojuan, Li Zesong, Tang Aifa, Gui Yaoting, Cai Zhiming
Abstract excerpt
BACKGROUND: Unprecedented progresses in high-throughput DNA sequencing and de novo gene synthesis technologies have allowed us to create living organisms in the absence of natural template. METHODOLOGY/PRINCIPAL FINDINGS: The sequence of wild-type S13 phage genome was downloaded from GenBank. Two synonymous mutations were introduced into wt-S13 genome to generate m1-S13 genome. Another mutant, m2-S13 genome, was...
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