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Article

Generation of gene-of-interest double allele knockout clones in primary human T cells by CRISPR.

STAR protocols - 15 Sept 2023

Wu Ling, Tan Jia Chi, Gascoigne Nicholas R J

Abstract excerpt

Gene-of-interest (GOI) knockout is an important technique to study the genetic mechanisms of T cells. Here, we present a protocol to generate GOI double allele gene knockouts in primary human T cells by CRISPR, thus depleting proteins of interest expressed intracellularly or extracellularly in primary T cells. We describe steps for gRNA selection and efficiency validation, homology-directed repair (HDR) DNA...

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Identifiers

PMID: 37432856
DOI: 10.1016/j.xpro.2023.102445
PMCID: PMC10362176

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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