Blocker Primer-Assisted LAMP Coupled with Pyrococcus furiosus Argonaute Enables Specific and Sensitive Detection of EGFR Mutations in NSCLC.

Abstract excerpt

Sensitive and specific detection of low-frequency mutations in circulating tumor DNA (ctDNA) remains a major challenge because mutant alleles typically constitute less than 1% of the total cell-free DNA in plasma. Herein, we present a blocker primer-assisted loop-mediated isothermal amplification (LAMP) assay coupled with Pyrococcus furiosus Argonaute (PfAgo) for detection of EGFR mutations associated with...

Topics

• Pyrococcus furiosus
• Humans
• Lung Neoplasms
• Mutation
• ErbB Receptors
• Nucleic Acid Amplification Techniques