Pyrococcus furiosus Argonaute-Based Fluorometric Biosensor for One-Tube Detection of Cancer-Associated Single Nucleotide Polymorphisms in MicroRNAs.

doi:10.1021/acs.analchem.4c07109

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Pyrococcus furiosus Argonaute-Based Fluorometric Biosensor for One-Tube Detection of Cancer-Associated Single Nucleotide Polymorphisms in MicroRNAs.

Analytical chemistry - 4 Mar 2025

Su Fengli, Zhao Wentao, Zhao Furong, Cao Min, Zhu Tianjiao, Lv Wei, Li Bingzhi

Abstract excerpt

MicroRNA-related single nucleotide polymorphisms (miR-SNPs) are promising biomarkers for cancer diagnostics, yet accurate detection methods remain limited. Here, we introduce a ligation-triggered Pyrococcus furiosus Argonaute (PfAgo) cleavage (LTAC) strategy for the sensitive detection of miR-SNPs, demonstrated using the rs11614913 SNP in miR-196a2, which is associated with nonsmall cell lung cancer (NSCLC). The...

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PMID: 39982863
DOI: 10.1021/acs.analchem.4c07109

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