Article
Multiple mutations in RNA polymerase β-subunit gene (rpoB) in Streptomyces incarnatus NRRL8089 enhance production of antiviral antibiotic sinefungin: modeling rif cluster region by density functional theory.
Bioscience, biotechnology, and biochemistry - 24 Apr 2021
Ogawa Saori, Shimidzu Hitomi, Fukuda Koji, Tsunekawa Naoki, Hirano Toshiyuki, Sato Fumitoshi, Yura Kei, Hasunuma Tomohisa, Ochi Kozo, Yamamoto Michio, Sakamoto Wataru, Hashimoto Kentaro, Ogata Hiroyuki, Kanao Tadayoshi, Nemoto Michiko, Inagaki Kenji, Tamura Takashi
Abstract excerpt
Streptomyces incarnatus NRRL8089 produces the antiviral, antifungal, antiprotozoal nucleoside antibiotic sinefungin. To enhance sinefungin production, multiple mutations were introduced to the rpoB gene encoding RNA polymerase (RNAP) β-subunit at the target residues, D447, S453, H457, and R460. Sparse regression analysis using elastic-net lasso-ridge penalties on previously reported H457X mutations identified a...
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