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Article

Site-specific insertion of spin-labeled L-amino acids in Xenopus oocytes.

Biochemistry - 6 Jul 2004

Shafer Aaron M, Kálai Tamás, Bin Liu Sarah Qiao, Hideg Kálmán, Voss John C

Abstract excerpt

Site-specific insertion of modified amino acids in proteins expressed in living cells is an emerging field holding great promise for elucidating protein structure-function relationships, expression levels, localization, and activation states in a complex milieu. To evaluate the efficiency of amino acids modified to carry either a nitroxide spin probe or a fluorescence probe, we have developed a screen using the...

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PMID: 15222758
DOI: 10.1021/bi035542i

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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