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Article

One-step purification and immobilization of recombinant proteins using SpyTag/SpyCatcher chemistry.

Biotechnology letters - 1 May 2021

Tian Junwei, Jia Ruiqi, Wenge Dong, Sun Hongxu, Wang Yue, Chang Yanhong, Luo Hui

Abstract excerpt

Based on the specific and spontaneous formation of isopeptide bonds by SpyCatcher/SpyTag, we have developed a one-step method for purification and immobilization of recombinant proteins. The procedure is to immobilize SpyCatcher on glyoxyl agarose gels, and then the SpyCatcher immobilisate can be used to immobilize the SpyTag-fused protein in the crude extract selectively. A mutant of SpyCatcher (mSC), in which a...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33591462
DOI: 10.1007/s10529-021-03098-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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