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Article

New strategy for reversible modulation of protein activity through site-specific conjugation of small molecule and polymer.

Bioconjugate chemistry - 16 Jul 2014

Wang Lei, Yuan Lin, Wang Hongwei, Liu Xiaoli, Li Xinming, Chen Hong

Abstract excerpt

A new strategy for accurate and reversible modulation of protein activity via simple conjugation of the sulfhydryl modifier and polymer with the introduced Cys residue in protein was developed in this study. With Escherichia coli inorganic pyrophosphatase (PPase) as a model protein, we used site-directed mutagenesis to generate a mutant PPase (PPC) with a substituted Cys residue at the specific Lys-148 site,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 24971741
DOI: 10.1021/bc5000934

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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