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Article

The TRACE-Seq method tracks recombination alleles and identifies clonal reconstitution dynamics of gene targeted human hematopoietic stem cells.

Nature communications - 20 Jan 2021

Sharma Rajiv, Dever Daniel P, Lee Ciaran M, Azizi Armon, Pan Yidan, Camarena Joab, Köhnke Thomas, Bao Gang, Porteus Matthew H, Majeti Ravindra

Abstract excerpt

Targeted DNA correction of disease-causing mutations in hematopoietic stem and progenitor cells (HSPCs) may enable the treatment of genetic diseases of the blood and immune system. It is now possible to correct mutations at high frequencies in HSPCs by combining CRISPR/Cas9 with homologous DNA donors. Because of the precision of gene correction, these approaches preclude clonal tracking of gene-targeted HSPCs....

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33473139
DOI: 10.1038/s41467-020-20792-y
PMCID: PMC7817666

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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