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Article

In Vivo Tracking of Hematopoietic Stem and Progenitor Cell Ontogeny by Cellular Barcoding.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Tak Tamar, Eisele Almut S, Perié Leïla

Abstract excerpt

Cellular barcoding is a powerful technique that allows for high-throughput mapping of the fate of single cells, notably hematopoietic stem and progenitor cells (HSPCs) after transplantation. Unique artificial DNA fragments, termed barcodes, are stably inserted into HSPCs using lentiviral transduction, making sure that each individual cell receives a single unique barcode. Barcoded HSPCs are transplanted into...

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PMID: 34057730
DOI: 10.1007/978-1-0716-1425-9_21

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