Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole-Genome Sequencing.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2021

Liu Guanqing, Qi Yiping, Zhang Tao

Abstract excerpt

The CRISPR/Cas systems have become the most widely used tool for genome editing in plants and beyond. However, CRISPR/Cas systems may cause unexpected off-target mutations due to sgRNA recognizing highly homologous DNA sequence elsewhere in the genome. Whole-genome sequencing (WGS) can be used to identify on- and off-target mutation. Here, we describe a pipeline of analyzing WGS data using a series of open source...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to CRISPR-Cas Systems
Share to Gene Editing
Share to Gene Transfer Techniques

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Vectors

Share to Transformation, Genetic

Share to Transgenes

Share to Whole Genome Sequencing

Source

Identifiers

PMID: 33471330
DOI: 10.1007/978-1-0716-1068-8_10

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Analysis of Off-Target Mutations in CRISPR-Edited Rice Plants Using Whole-Genome Sequencing.PMID 33471330Read ↗

A large-scale whole-genome sequencing analysis reveals highly specific genome editing by both Cas9 and Cpf1 (Cas12a) nucleases in rice.Semantic proximity Read ↗

The CRISPR/Cas9 system produces specific and homozygous targeted gene editing in rice in one generation.Semantic proximity Read ↗

Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism.Semantic proximity Read ↗

Hi-TOM: a platform for high-throughput tracking of mutations induced by CRISPR/Cas systemsSemantic proximity Read ↗

Genome editing in rice and wheat using the CRISPR/Cas system.Semantic proximity Read ↗

SuperDecode: An integrated toolkit for analyzing mutations induced by genome editing.Semantic proximity Read ↗

Expanding Gene-Editing Potential in Crop Improvement with Pangenomes.Semantic proximity Read ↗

Effective identification of CRISPR/Cas9-induced and naturally occurred mutations in rice using a multiplex ligation-dependent probe amplification-based method.Semantic proximity Read ↗

Genotyping genome-edited mutations in plants using CRISPR ribonucleoprotein complexes.Semantic proximity Read ↗

A Robust CRISPR/Cas9 System for Convenient, High-Efficiency Multiplex Genome Editing in Monocot and Dicot Plants.Semantic proximity Read ↗

On- and Off-Target Analyses of CRISPR-Cas12b Genome Editing Systems in Rice.Semantic proximity Read ↗

CRISPR/Cas9: a promising way to exploit genetic variation in plants.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.