Article
Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism.
Plant cell reports - 1 Jul 2016
Zheng Xuelian, Yang Shixin, Zhang Dengwei, Zhong Zhaohui, Tang Xu, Deng Kejun, Zhou Jianping, Qi Yiping, Zhang Yong
Abstract excerpt
KEY MESSAGE: A method based on DNA single-strand conformation polymorphism is demonstrated for effective genotyping of CRISPR/Cas9-induced mutants in rice. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) has been widely adopted for genome editing in m...
Topics
- Base Sequence
- CRISPR-Cas Systems
- Gene Frequency
- Genotype
- Genotyping Techniques
- INDEL Mutation
- Models, Genetic
- Mutagenesis, Site-Directed
- Oryza
- Plant Proteins
- Polymorphism, Single-Stranded Conformational
- Reproducibility of Results