Article
Effective screen of CRISPR/Cas9-induced mutants in rice by single-strand conformation polymorphism.
Plant cell reports - 1 Jul 2016
Zheng Xuelian, Yang Shixin, Zhang Dengwei, Zhong Zhaohui, Tang Xu, Deng Kejun, Zhou Jianping, Qi Yiping, Zhang Yong
Abstract excerpt
KEY MESSAGE: A method based on DNA single-strand conformation polymorphism is demonstrated for effective genotyping of CRISPR/Cas9-induced mutants in rice. Clustered regularly interspaced short palindromic repeats (CRISPR)/CRISPR-associated 9 (Cas9) has been widely adopted for genome editing in many organisms. A large proportion of mutations generated by CRISPR/Cas9 are very small insertions and deletions...
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