Article
FRET and optical trapping reveal mechanisms of actin activation of the power stroke and phosphate release in myosin V.
The Journal of biological chemistry - 18 Dec 2020
Gunther Laura K, Rohde John A, Tang Wanjian, Cirilo Joseph A, Marang Christopher P, Scott Brent D, Thomas David D, Debold Edward P, Yengo Christopher M
Abstract excerpt
Myosins generate force and motion by precisely coordinating their mechanical and chemical cycles, but the nature and timing of this coordination remains controversial. We utilized a FRET approach to examine the kinetics of structural changes in the force-generating lever arm in myosin V. We directly compared the FRET results with single-molecule mechanical events examined by optical trapping. We introduced a...
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