Article
Switch 1 mutation S217A converts myosin V into a low duty ratio motor.
The Journal of biological chemistry - 23 Jan 2009
Forgacs Eva, Sakamoto Takeshi, Cartwright Suzanne, Belknap Betty, Kovács Mihály, Tóth Judit, Webb Martin R, Sellers James R, White Howard D
Abstract excerpt
We have determined the kinetic mechanism and motile properties of the switch 1 mutant S217A of myosin Va. Phosphate dissociation from myosin V-ADP-Pi (inorganic phosphate) and actomyosin V-ADP-Pi and the rate of the hydrolysis step (myosin V-ATP-->myosin V-ADP-Pi) were all approximately 10-fold slower in the S217A mutant than in wild type (WT) myosin V, resulting in a slower steady-state rate of basal and...
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