Article
Transglutaminase 2 crosslinks the glutathione S-transferase tag, impeding protein-protein interactions of the fused protein.
Experimental & molecular medicine - 1 Jan 2021
Kim Hyo-Jun, Lee Jin-Haeng, Lee Ki Baek, Shin Ji-Woong, Kwon Mee-Ae, Lee Soojin, Jeong Eui Man, Cho Sung-Yup, Kim In-Gyu
Abstract excerpt
Glutathione S-transferase (GST) from Schistosoma japonicum has been widely used as a tag for affinity purification and pulldown of fusion proteins to detect protein-protein interactions. However, the reliability of this technique is undermined by the formation of GST-fused protein aggregates afte...
Topics
- Binding Sites
- Cross-Linking Reagents
- Glutathione Transferase
- HEK293 Cells
- HeLa Cells
- Humans
- Mutation
- Protein Binding
- Protein Glutamine gamma Glutamyltransferase 2
- Recombinant Proteins
- Tumor Suppressor Protein p53