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Article

Necessity for Validation of Effectiveness of Selected Guide RNA In Silico for Application of CRISPR/Cas9.

Molecular biotechnology - 1 Feb 2021

Kim Dong-Hwan, Lee Joonbum, Suh Yeunsu, Lee Kichoon

Abstract excerpt

Selection of guide RNA (gRNA) is important to increase the efficiency of gene editing in the CRISPR/Cas9 system. Due to the variation in actual efficiency of insertion/deletion (indel) mutation among selected gRNAs in silico, reliable methods for validation of efficiency of gRNA need to be developed. Three gRNAs with high on-target scores (72.0 for target 1, 65.4 for target 2, and 62.9 for target 3) were designed...

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Identifiers

PMID: 33386580
DOI: 10.1007/s12033-020-00290-8

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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