Article

Targeted Deletion of Vitamin D receptor Gene in Mammalian Cells by CRISPR/Cas9 Systems

2016-02-18

Abstract excerpt

CRISPR/Cas9 system has become a new versatile technology for genome engineering. It utilizes a single guide RNA (sgRNA) to recognize target sequences in genome function, and activates Cas9 endonucleases to cut the locus. In this study, we designed two target sites from conserved regions of vitamin D receptor ( VDR ) gene in mammalian cells, which cover more than 17 kb of chromosome region depending on the species...

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Literature Corpus work: 3b4f1afd-92f4-5486-aac1-54621a9a5d8e
DOI: 10.1101/040147

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