Article
All-in-one approaches for rapid and highly specific quantifcation of single nucleotide polymorphisms based on ligase detection reaction using molecular beacons as turn-on probes.
Talanta - 1 Mar 2021
Zhang Wancun, Liu Kangbo, Zhang Pin, Cheng Weyland, Zhang Yaodong, Li Linfei, Yu Zhidan, Chen Mengmeng, Chen Lin, Li Lifeng, Zhang Xianwei
Abstract excerpt
Rapid, simple, specific and sensitive approaches for single nucleotide polymorphisms (SNPs) detection are essential for clinical diagnosis. In this study, all-in-one approaches, consisting of the whole detection process including ligase detection reaction (LDR) and real time quantitative polymerase chain reaction performed in one PCR tube by a one-step operation on a real-time PCR system using molecular beacon...
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