Article
A cell cycle-dependent CRISPR-Cas9 activation system based on an anti-CRISPR protein shows improved genome editing accuracy.
Communications biology - 23 Oct 2020
Matsumoto Daisuke, Tamamura Hirokazu, Nomura Wataru
Abstract excerpt
The development of genome editing systems based on the Cas9 endonuclease has greatly facilitated gene knockouts and targeted genetic alterations. Precise editing of target genes without off-target effects is crucial to prevent adverse effects in clinical applications. Although several methods hav...
Topics
- Binding Sites
- CRISPR-Associated Protein 9
- CRISPR-Associated Proteins
- CRISPR-Cas Systems
- Cell Cycle
- Cell Line
- Gene Editing
- Gene Expression Regulation
- Humans
- Mutation
- Plasmids
- RNA, Guide, CRISPR-Cas Systems