Article
Timed inhibition of CDC7 increases CRISPR-Cas9 mediated templated repair.
Nature communications - 30 Apr 2020
Wienert Beeke, Nguyen David N, Guenther Alexis, Feng Sharon J, Locke Melissa N, Wyman Stacia K, Shin Jiyung, Kazane Katelynn R, Gregory Georgia L, Carter Matthew A M, Wright Francis, Conklin Bruce R, Marson Alex, Richardson Chris D, Corn Jacob E
Abstract excerpt
Repair of double strand DNA breaks (DSBs) can result in gene disruption or gene modification via homology directed repair (HDR) from donor DNA. Altering cellular responses to DSBs may rebalance editing outcomes towards HDR and away from other repair outcomes. Here, we utilize a pooled CRISPR screen to define host cell involvement in HDR between a Cas9 DSB and a plasmid double stranded donor DNA (dsDonor). We find...
Topics
- CRISPR-Cas Systems
- Cell Cycle Proteins
- DNA Breaks, Double-Stranded
- Gene Editing
- Genetic Engineering
- HCT116 Cells
- HEK293 Cells
- HeLa Cells
- Homologous Recombination
- Humans