[Enhanced thermostability of Rhizopus chinensis lipase by error-prone PCR].

Article

[Enhanced thermostability of Rhizopus chinensis lipase by error-prone PCR].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Dec 2013

Wang Rui, Yu Xiaowei, Xu Yan

Abstract excerpt

Directed evolution was conducted to improve the thermostability of lipase from Rhizopus chinensis CCTCC M201021. Mutations were introduced by two rounds of error-prone PCR and mutant lipase was selected by fast-blue RR top agar screening. Two positive variants were selected in the first-round and...

Topics

Cloning, Molecular
Directed Molecular Evolution
Enzyme Stability
Hot Temperature
Industrial Microbiology
Lipase
Mutation
Pichia
Polymerase Chain Reaction
Protein Engineering
Rhizopus

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