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Article

[Enhanced thermostability of Rhizopus chinensis lipase by error-prone PCR].

Sheng wu gong cheng xue bao = Chinese journal of biotechnology - 1 Dec 2013

Wang Rui, Yu Xiaowei, Xu Yan

Abstract excerpt

Directed evolution was conducted to improve the thermostability of lipase from Rhizopus chinensis CCTCC M201021. Mutations were introduced by two rounds of error-prone PCR and mutant lipase was selected by fast-blue RR top agar screening. Two positive variants were selected in the first-round and four in the second-round screening process. Ep2-4 was proved as the most thermostable lipase and its DNA sequencing...

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PMID: 24660623

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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