Article
Performance comparison of deep sequencing platforms for detecting HIV-1 variants in the pol gene.
Journal of medical virology - 1 Sept 2018
Nicot Florence, Jeanne Nicolas, Raymond Stéphanie, Delfour Olivier, Carcenac Romain, Lefebvre Caroline, Sauné Karine, Delobel Pierre, Izopet Jacques
Abstract excerpt
The present study compares the performances of an in-house sequencing protocol developed on MiSeq, the Sanger method, and the 454 GS-FLX for detecting and quantifying drug-resistant mutations (DRMs) in the human immunodeficiency virus polymerase gene (reverse transcriptase [RT] and protease [PR]). MiSeq sequencing identified all the resistance mutations detected by bulk sequencing (n = 84). Both the MiSeq and 454...
Topics
- Drug Resistance, Viral
- Genetic Variation
- HIV Infections
- HIV Protease
- HIV Reverse Transcriptase
- HIV-1
- High-Throughput Nucleotide Sequencing
- Humans
- Mutation
- pol Gene Products, Human Immunodeficiency Virus