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Article

Format chain exchange (FORCE) for high-throughput generation of bispecific antibodies in combinatorial binder-format matrices.

Nature communications - 2 Oct 2020

Dengl Stefan, Mayer Klaus, Bormann Felix, Duerr Harald, Hoffmann Eike, Nussbaum Bianca, Tischler Michael, Wagner Martina, Kuglstatter Andreas, Leibrock Lea, Buldun Can, Georges Guy, Brinkmann Ulrich

Abstract excerpt

Generation of bispecific antibodies (bsAbs) requires a combination of compatible binders in formats that support desired functionalities. Here, we report that bsAb-matrices can be generated by Format Chain Exchange (FORCE), enabling screening of combinatorial binder/format spaces. Input molecules for generation of bi/multi-valent bsAbs are monospecific entities similar to knob-into-hole half-antibodies, yet with...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 33009381
DOI: 10.1038/s41467-020-18477-7
PMCID: PMC7532213

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

Source receipt: pubmed, retrieved 6 Sept 2026.

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