Article
Somatic mutation detection efficiency in EGFR: a comparison between high resolution melting analysis and Sanger sequencing.
BMC cancer - 22 Sept 2020
Joy Reenu Anne, Thelakkattusserry Sukrishna Kamalasanan, Vikkath Narendranath, Bhaskaran Renjitha, Krishnan Sajitha, Vasudevan Damodaran, Ariyannur Prasanth S
Abstract excerpt
BACKGROUND: High resolution melting curve analysis is a cost-effective rapid screening method for detection of somatic gene mutation. The performance characteristics of this technique has been explored previously, however, analytical parameters such as limit of detection of mutant allele fraction and total concentration of DNA, have not been addressed. The current study focuses on comparing the mutation detection...
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