Article
Melting curve analysis for mutations of EGFR and KRAS.
Anticancer research - 1 Feb 2014
Furugaki Koh, Yasuno Hideyuki, Iwai Toshiki, Moriya Yoichiro, Harada Naoki, Fujimoto-Ouchi Kaori
Abstract excerpt
Epidermal growth factor receptor (EGFR) and v-Ki-ras2 Kirsten rat sarcoma viral oncogene homolog (KRAS) mutations are common in non-small cell lung cancer (NSCLC) and colorectal cancer (CRC). The aim of the present study was to develop a simple and versatile tool to determine EGFR and KRAS mutations for pre-clinical research in the laboratory. We developed a melting curve analysis to detect exon 19 deletion,...
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