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Article

Enhanced DNA dynamics due to cationic reagents, topological states of dsDNA and high mobility group box 1 as probed by PicoGreen.

The FEBS journal - 1 Jan 2009

Noothi Sunil K, Kombrabail Mamata, Kundu Tapas K, Krishnamoorthy G, Rao Basuthkar J

Abstract excerpt

We report a study of dynamics with a dsDNA-specific dye called PicoGreen bound to plasmid DNA (3.4 kb), and show that at low dye/DNA phosphate ratios (1 : 100 and below), PicoGreen dynamics reflect the motional dynamics of dsDNA. We further evaluated the usefulness of this probe by measuring the time-resolved fluorescence dynamics of PicoGreen bound to dsDNA in the presence of cationic reagents that affect DNA...

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Identifiers

PMID: 19087192
DOI: 10.1111/j.1742-4658.2008.06802.x

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HMGB1

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