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Article

Construction, Investigation and Application of TEV Protease Variants with Improved Oxidative Stability.

Journal of microbiology and biotechnology - 28 Dec 2021

Bayar Enkhtuya, Ren Yuanyuan, Chen Yinghua, Hu Yafang, Zhang Shuncheng, Yu Xuelian, Fan Jun

Abstract excerpt

Tobacco etch virus protease (TEVp) is a useful tool for removing fusion tags, but wild-type TEVp is less stable under oxidized redox state. In this work, we introduced and combined C19S, C110S and C130S into TEVp variants containing T17S, L56V, N68D, I77V and S135G to improve protein solubility, and S219V to inhibit self-proteolysis. The solubility and cleavage activity of the constructed variants in Escherichia...

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Identifiers

PMID: 34528919
DOI: 10.4014/jmb.2106.06075
PMCID: PMC9705859

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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